N-Acetyl-D-galactosamine-Specific Lectin Isolation from Soyflour with Poly(HPMA-GMA) Beads

Percin I., Yavuz H., Aksoz E., DENİZLİ A.

JOURNAL OF APPLIED POLYMER SCIENCE, vol.111, no.1, pp.148-154, 2009 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 111 Issue: 1
  • Publication Date: 2009
  • Doi Number: 10.1002/app.29054
  • Journal Name: JOURNAL OF APPLIED POLYMER SCIENCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.148-154
  • Hacettepe University Affiliated: Yes

Abstract

Soybean lectin was purified from seeds of Glycine max L.Merrill SA88. Poly(hydroxypropyl methacrylate-glycidyl methacrylate) [poly(HPMA-GMA)l beads were used as an affinity matrix and N-acetyl-D-galactosamine (GalNAc) was used as an affinity ligand. Soybean lectin adsorption with GalNAc attached poly(HPMA-GMA) beads from soybean lectin solution (in phosphate buffered saline) was 5.0 mg/g. Maximum adsorption capacity for soybean lectin from the soy flour extract was 26.0 mg/g. Elution of soybean lectin from adsorbent was accomplished by 0.5M galactose solution. Purity of soybean lectin was determined by SDS-PAGE. It was observed that soybean lectin could be repeatedly adsorbed and desorbed with GalNAc-attached poly(HPMA-GMA) beads. (C) 2008 Wiley Periodicals, Inc. J Appl Polym Sci 111: 148-154, 2009