Surface-enhanced Raman scattering-based detection of plasmin activity by specific peptide substrate

Yazğan N. N., Bulat T., Topcu A., Dudak F. C., Boyaci I. H., Tamer U.

FOOD CHEMISTRY, cilt.372, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 372
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.foodchem.2021.131235
  • Dergi Adı: FOOD CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Plasmin activity, SERS, Peptide substrate, Milk, DAIRY-PRODUCTS, MILK, ENZYME, ASSAY, SPECTROSCOPY, PROTEASE, PLATFORM, BINDING, CITRATE, CASEIN
  • Hacettepe Üniversitesi Adresli: Evet

Özet

In this study, a new surface-enhanced Raman scattering (SERS)-based method has been developed for the detection of plasmin activity. Firstly, different peptide sequences, which are specific to plasmin, were examined. Then, SERS substrates were prepared by chosen peptide substrate. Enzyme activity was determined by pursuing the reduction of DTNB band at 1331 cm-1 with Raman spectroscopy. The reduction in SERS intensity was related to the plasmin activity, and changes in SERS intensity vs. plasmin concentration graph was obtained. Limit of detection (LOD) and limit of quantification (LOQ) values were calculated as 2.14 U/mL and 6.42 U/mL, respectively. Intra-and inter-day repeatability results were determined as 1.45% and 1.47% relative standard deviation (RSD). Also, recovery of the method was determined for the plasmin spiked milk samples. The results demonstrated that the proposed method could be successfully used to detect the plasmin activity in milk samples.