A generic procedure to monitor Maillard-derived fluorescent compounds in cookies by flow-injection analysis


Calvarro J., GÖKMEN V. , Morales F. J.

EUROPEAN FOOD RESEARCH AND TECHNOLOGY, cilt.229, sa.6, ss.843-851, 2009 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 229 Konu: 6
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1007/s00217-009-1119-8
  • Dergi Adı: EUROPEAN FOOD RESEARCH AND TECHNOLOGY
  • Sayfa Sayıları: ss.843-851

Özet

Flow-injection analysis is proposed for routine measurement of Maillard-derived fluorescent compounds (FC) in cookies as a marker of the extent of baking. In addition, procedure was applied to investigate the formation of free and total (bound to protein free) FC in cookie-resembling models and in commercial wheat-based cookies as well. FC accounts for the overall fluorescence response of Maillard-derived fluorescent compounds (bound or not to protein) formed during baking but not for a single compound. Free and total FC values increased exponentially during baking at 200, 210 and 220 A degrees C and an induction period of 10 min was observed for free FC. In the complex scenario of the progress of the Maillard reaction (MR) during baking, formation of FC (347/415, ext/emm) was not the limiting step for browning development at the advanced stage of the reaction. Furthermore, the formation of Maillard-derived fluorescent compounds and browning during baking were a consequence of parallel reactions apart from the classical MR scheme of consecutive reactions. Total/free FC ratio was dependent on the baking conditions applied and ratio was significantly decreased at severe baking conditions. Total/free FC ratio could be used as a reference marker for monitoring the process and to identify potential over-processing situations during baking. In addition, fluorescent residues were originally bound to protein because total/free FC ratio decreased drastically as increased the temperature and time of the process. Levels of total FC were nearly 20-fold of free FC in commercial samples. Values of FC were positively correlated with acrylamide, a Maillard-derived food processing contaminant.