Applications of Bacteriophage Cocktails to Reduce Salmonella Contamination in Poultry Farms


Evran S., Tayyarcan E. K., Acar-Soykut E., BOYACI İ. H.

FOOD AND ENVIRONMENTAL VIROLOGY, vol.14, no.1, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 14 Issue: 1
  • Publication Date: 2022
  • Doi Number: 10.1007/s12560-021-09501-0
  • Journal Name: FOOD AND ENVIRONMENTAL VIROLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Keywords: Salmonella, Bacteriophage, Biocontrol, Microbial contamination, Disinfection, PHAGE, COLONIZATION, SURFACES, BIOFILMS
  • Hacettepe University Affiliated: Yes

Abstract

Salmonella contamination is a critical problem in poultry farms, with serious consequences for both animals and food products. The aim of this study is to investigate the use of phage cocktails to reduce Salmonella contamination in poultry farms. Within the scope of the study, Salmonella phages were isolated from chicken stool. After the host range of phages was determined, morphological characterization was performed through transmission electron microscopy analysis. Then, replication parameters and adsorption rates were determined by one-step growth curves. After that, phage cocktail was prepared, and its effectiveness was tested in three environments, which were drinking water, shavings, and plastic surfaces. The results obtained have demonstrated that the phage cocktail can reduce Salmonella count up to 2.80 log(10) units in drinking water, up to 2.30 log(10) units on shavings, and 2.31 log(10) units on plastic surfaces. It has been determined that phage cocktails could be a successful alternative in reducing Salmonella contamination in poultry environment. This work is the first study to investigate the use of phage cocktails for reducing Salmonella contamination in poultry water and on shavings, and it is presumed that the results obtained will contribute to the fight against pathogens by making them applicable to poultry farms.