Akademik Veri Yönetim Sistemi
Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Hacettepe Üniversitesi, Sağlık Bilimleri Enstitüsü, Kök Hücre Bilimleri A.B.D., Türkiye
Tezin Onay Tarihi: 2025
Tezin Dili: İngilizce
Öğrenci: ŞİMAL ŞENOCAK
Danışman: Fatima Susanna F. Aerts Kaya
Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
Özet:
Although the umbilical cord is usually disposed of as medical waste after birth, it is considered an important resource due to the biological potential it offers in the field of regenerative medicine. Embryonic stem cells (ESCs) create ethical concerns despite having the potential to differentiate into all cell types. In contrast, induced pluripotent stem cells (iPSCs), derived from adult somatic cells, do not have such ethical issues since they are developed from somatic cells. However, iPSCs have different epigenetic memories depending on the tissue they are isolated and developed from, and therefore their (spontaneous) differentiation properties may vary from each other. In addition, the quality and characteristics of iPSCs may vary depending on age, gender and disease status. Within the scope of this thesis, mesodermal-derived Wharton Jelly mesenchymal stem cells (WJ-MSCs) and ectodermal-derived cord outer lining epithelial cells (CLECs) were isolated and characterized from the extra-embryonal umbilical cord. Comparisons were made with bone marrow mesenchymal stem cells (BM-MSC), which are frequently used as an iPSC source during characterization. Lentiviral OSKM vector was used for the development of iPSC from CLEC and WJ-MSCs. The pluripotency of the developed WJ-MSC and CLEC iPSC clones was evaluated by RT-PCR. As a result, it was determined that CLEC cultures consisted of a more homogeneous cell population with advancing passages. CLECs morphologically resemble epithelial cells and show pan-cytokeratin surface expression. WJ-MSC and CLEC iPSC clones produced after reprogramming express pluripotency genes OCT4, SOX2 and NANOG.