An efficient method for stable transfection of mouse myogenic C2C12 cell line using a nonviral transfection approach

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Akyuz M. D., Hayta B., Dincer P. R.

TURKISH JOURNAL OF MEDICAL SCIENCES, cilt.41, sa.5, ss.821-825, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 5
  • Basım Tarihi: 2011
  • Doi Numarası: 10.3906/sag-1008-1057
  • Dergi Adı: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.821-825
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Aim: To describe in detail the use of the T-REx (TM) System (a tetracycline-regulated mammalian expression system; Invitrogen) for rapid, easy, and effective generation of stable C2C12 cell clones. Tetracycline-inducible stable cell lines are useful tools to study the function of various genes in different types of mammalian cells. However, the generation of stable cell lines is a time-consuming, technically difficult, and expensive process.