An efficient method for stable transfection of mouse myogenic C2C12 cell line using a nonviral transfection approach

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Akyuz M. D., Hayta B., Dincer P. R.

TURKISH JOURNAL OF MEDICAL SCIENCES, vol.41, no.5, pp.821-825, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 5
  • Publication Date: 2011
  • Doi Number: 10.3906/sag-1008-1057
  • Journal Name: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.821-825
  • Hacettepe University Affiliated: Yes

Abstract

Aim: To describe in detail the use of the T-REx (TM) System (a tetracycline-regulated mammalian expression system; Invitrogen) for rapid, easy, and effective generation of stable C2C12 cell clones. Tetracycline-inducible stable cell lines are useful tools to study the function of various genes in different types of mammalian cells. However, the generation of stable cell lines is a time-consuming, technically difficult, and expensive process.