Molecularly imprinted based surface plasmon resonance nanosensors for microalbumin detection

Esenturk M. K., AKGÖNÜLLÜ S., Yilmaz F., DENİZLİ A.

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, vol.30, no.8, pp.646-661, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 8
  • Publication Date: 2019
  • Doi Number: 10.1080/09205063.2019.1600181
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.646-661
  • Keywords: Microalbumin detection, nanoparticles, surface plasmon resonance, nanosensor, HUMAN SERUM-ALBUMIN, NANOPARTICLES, BIOSENSOR, PROTEIN, URINE, LABEL, ASSAY, NANOFILMS
  • Hacettepe University Affiliated: Yes


Human serum albumin (HSA) is a major blood plasma protein also found in urine where its existence may be a marker of some types of liver or kidney dysfunction. Herein, we fabricated a novel surface plasmon resonance (SPR) nanosensor for selective, sensitive, and label-free microalbumin detection both in aqueous and urine sample solutions. First, HSA-imprinted nanoparticles were synthesized, which consist of ethylene glycol dimethacrylate and N-methacryloyl-L-leucine methyl ester as a cross-linker and functional monomer. The nanoparticles were characterized by zeta-size and scanning electron microscope analyses and were dropped onto the SPR chip surface to make HSA sensitive nanosensor. Characterization studies of HSA-imprinted SPR chip were carried out by atomic force microscopy, Fourier-transform infrared spectroscopy, contact angle, and ellipsometer. The limit of detection and limit of quantification values of HSA-imprinted SPR nanosensor were calculated as 0.7pM and 1.9pM for the concentration range of 0.15-500nM. Selectivity studies of HSA-imprinted SPR nanosensor were achieved with hemoglobin and transferrin proteins which were chosen as competitor molecules. HSA-imprinted SPR nanosensor was displayed highly selective and sensitive to HSA.