Prevalence of O25b-ST131 clone and fosfomycin resistance in urinary Escherichia coli isolates and their relation to CTX-M determinant

Demirci-Duarte S., Unalan-Altintop T., Koseoglu E., Cakar A., Altun B., Sancak B., ...More

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, vol.98, no.1, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 98 Issue: 1
  • Publication Date: 2020
  • Doi Number: 10.1016/j.diagmicrobio.2020.115098
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Keywords: Fosfomycin, ST131 clone, H30-R/H30-Rx subclone, Beta-lactamases, MULTIDRUG-RESISTANT, GENE FOSA3, ENTEROBACTERIACEAE, EPIDEMIOLOGY, INFECTIONS
  • Hacettepe University Affiliated: Yes


Escherichia coli ST131 clone and H30-R/H30-Rx subclones are the most common multidrug-resistant high-risk clones in Wis. Antimicrobial susceptibility of fosfomycin was compared to five other agents in consecutively collected 299 urinary isolates using the agar dilution method. Prevalence of the ST131 clone and the occurrence of blaCTX-M were also investigated. Overall resistance to fosfomycin, cefuroxime, and ceftriaxone were 2.7%, 35.4%, and 30.1% respectively. fosA, fosA3, and fosC.2 genes were not detected. In isolates resistant to ciprofloxacin (34.7%), the prevalence of ST131 clone was 31.7%, of which 81.8% belonged to H30-R and 66.7% to H30-Rx subclones. None of the isolates of the ST131 clone were resistant to fosfomycin. However, bla(CTX-M) occurred in 57.6% of the isolates among this clone, 62.9% in H30-R and 68.2% in H30-Rx subclones. The results of this study suggest that fosfomycin resistance is not prevalent in urinary isolates, however, bla(CTX-M) m positive ST131 clone is quite common. (C) 2020 Elsevier Inc. All rights reserved.