The ABILOCO is a questionnaire measuring mobility in stroke patients. The aim of the study was to evaluate the psychometric properties of the ABILOCO-Turkish. One hundred forty stroke patients were included. Thirteen items were rated according to 3 levels: difficult, easy, and impossible. The construct validity of the ABILOCO-Turkish was investigated using exploratory and confirmatory factor analysis. The reliability of the ABILOCO-Turkish was investigated through internal consistency approaches and test–retest reliability. The ABILOCO-Turkish was compared with the Timed Up & Go (TUG) test and the Berg Balance Scale for validity. The scale was repeated 1 week later for test–retest reliability. The Cronbach’s alpha value calculated was 0.96 and item–total score correlations were between 0.464 and 0.899. The intraclass correlation coefficient (ICC) calculated for test–retest reliability was 0.989. According to the results, the ABILOCO-Turkish is highly correlated with the TUG (r: −0.830, p < 0.001) and the Berg Balance Scale (r: 0.919, p < 0.001). The exploratory factor analysis of the ABILOCO-Turkish showed that it is unidimensional, with this factor explaining 66.56% of the variance, and the construct validity was proven by confirmatory factor analysis. The Turkish version of the ABILOCO is a reliable and valid scale for evaluating mobility in stroke patients.