Concanavalin A immobilized magnetic poly(glycidyl methacrylate) beads for prostate specific antigen binding

İDİL N., Percin I., KARAKOC V., Yavuz H., AKSÖZ N., DENİZLİ A.

COLLOIDS AND SURFACES B-BIOINTERFACES, cilt.134, ss.461-468, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 134
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1016/j.colsurfb.2015.06.050
  • Dergi Adı: COLLOIDS AND SURFACES B-BIOINTERFACES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.461-468
  • Anahtar Kelimeler: Concanavalin A, mPGMA beads, Prostate specific antigen, Prostate cancer, Spacer arm, SCREENING-TEST, HUMAN SERUM, CANCER, HYPERPLASIA, LECTIN, GLYCOSYLATION, PSA, NANOPARTICLES, MICROSPHERES, PARTICLES
  • Hacettepe Üniversitesi Adresli: Evet

Özet

The aim of this study was to prepare Concanavalin A (Con A) immobilized magnetic poly(glycidyl methacrylate) (mPGMA) beads for prostate specific antigen (PSA) binding and to study binding capacities of the beads using lectin glycoprotein interactions. Firstly, iron oxide nanoparticles were synthesized by co-precipitation method and then, beads were synthesized by dispersion polymerization in the presence of iron oxide nanoparticles. Con A molecules were both covalently immobilized onto the beads directly and through the spacer arm (1,6-diaminohexane-HDMA). The total PSA and free PSA binding onto the mPGMA-HDMA-Con A beads were higher than that of the mPGMA-Con A beads. Maximum PSA binding capacity was observed as 91.2 ng/g. Approximately 45% of the bound PSA was eluted by using 0.1 M mannose as elution agent. The mPGMA-HDMA-Con A beads could be reused without a remarkable decrease in the binding capacities after 5 binding-desorption cycles. Serum fractions were analyzed using SDS-PAGE. The mPGMA-HDMA-Con A beads could be useful for the detection of PSA and suggested as a model system for other glycoprotein biomarkers. (C) 2015 Elsevier B.V. All rights reserved.