Objectives To investigate the artefacts caused by different disinfection and protection methods that can be used for infection control of photostimulable phosphor (PSP) plates. Methods The plates that were enveloped with single or double envelopes were sprayed with an alcohol-containing solution or wiped with an alcohol-containing tissue. Four PSP groups with two plates in each group were formed (A = wiping single envelope, B = wiping double envelopes, C = spraying onto single envelope, and D = spraying onto double envelopes). Any artefacts (1 = no artefact, 2 = presence of artefact less than 0.5 cm wide, and 3 = presence of artefact larger than 0.5 cm wide) on the 12 acquired images were evaluated. Results Artefacts (score-3) occurred on the images of Group C-2 PSP plate after the 3rd exposure. According to the Kruskal-Wallis test the difference between the artefact score of the four methods was found significant. Post-hoc comparisons showed the Group C artefact scores were higher than the others and the difference was significant. The total time of enveloping and disinfection processes for groups was; A = 6.30 min, B = 7.58 min, C = 5.48 min, and D = 7.14 min. Conclusions Regardless of the number of envelopes, wiping with a tissue was less likely to cause artefacts, while spraying was reliable only when double envelopes were used. However, using a tissue and double envelopes, which are less risky in terms of artefact, causes time loss, difficulty in manipulation, environmental pollution and high cost.