Akademik Veri Yönetim Sistemi
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, cilt.23, sa.6, ss.579-593, 2008 (SCI-Expanded)
A simple, fast, inexpensive sensitive/selective nucleic acid sensor based on the quartz crystal microbalance system (QCM) was developed for the detection of telomerase. A specially designed oligonucleotide probe, that is complementary to the template region of the telomerase enzyme, was immobilized onto the silver electrodes of piezoelectric crystals with polyethyleneimine adhesion cross-linked with glutaraldehyde. Optimum coating and immobilization conditions were determined. The QCM nucleic acid sensor was used for the detection of telomerase enzyme in human cervix carcinoma cell (HeLa) lysates. A significant difference between the telomerase negative cells (BHK) from HeLa cells, which was used for qualitative and quantitative detection of telomerase. An almost a linear correlation with the protein concentration was obtained with a R-2 value of 0.9621. The linear correlation coefficients (R-2) for HeLa and BHK cell lysate obtained by applying Langmuir adsorption model, were 0.9630 and 0.9766, respectively. Telomerase activity was confirmed by stretch-PCR and fluorometric (F)-TRAP within HeLa extracts. The results obtained both with the QCM system developed in this study and F-TRAP method applied is significantly correlated (r = 0.968, p < 0.001).