A thermosensitive gel loaded with an enzyme and an antibiotic drug for the treatment of periprosthetic joint infection
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, cilt.43, ss.423-429, 2018 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 43
- Basım Tarihi: 2018
- Doi Numarası: 10.1016/j.jddst.2017.11.004
- Dergi Adı: JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.423-429
- Anahtar Kelimeler: Periprosthetic joint infection, Vancomycin hydrochloride, Serratiopeptidase, Thermosensitive gel, Drug delivery, Antibiofilm activity, IN-VITRO, BIOFILM RESISTANCE, CONTROLLED-RELEASE, DELIVERY-SYSTEM, VANCOMYCIN, HYDROGEL, FORMULATION, LIPOSOMES, GRAFTS
- Hacettepe Üniversitesi Adresli: Evet
Özet
Biofilms have an important role in the pathogenesis of Periprosthetic Joint Infection (PJI)s and are very likely the main cause in resistance to conventional treatments. In this study, a novel thermosensitive, Pluronic - based gel formulation, containing an enzyme (Serratiopeptidase, SP), an antibiotic drug (Vancomycin HCl, VA) and antibiotic-loaded microspheres (TG-EAM) were prepared and evaluated for their effect against PJI. Viscosity measurements, drug and enzyme release, in vitro enzyme activity and in vitro anti-biofilm activity studies were performed to characterize the formulations. While in vitro drug release studies showed that 74.5% VA was released after 25 d from TG-EAM, the in vitro enzyme activity was measurable for two days. Based on the obtained experimental results, the formulation containing 0.8% (w/v) free VA, 5% (w/v) SP and 40% (w/v) of VA loaded microspheres was chosen as the most suitable formulation for future in vivo studies, as a promising alternative to current standard treatments for PJI.