Akademik Veri Yönetim Sistemi
Materials Chemistry and Physics, cilt.296, 2023 (SCI-Expanded)
© 2022 Elsevier B.V.In this study, silica nanoparticles were synthesized via the well-known Stöber method and subsequently decorated with amino functional groups before the immobilization of recognizing element, the anti-transferrin antibody. Antibody immobilization was achieved by following N-ethyl-N’-(3-(dimethylamino)propyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) activation/coupling process while preventing protein denaturation as well as losing recognizing capability. After comprehensive characterization methods via scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDAX), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and zeta size/potential measurements, the factors affecting on target transferrin molecules were optimized by varying initial concentration, temperature, interaction time, and pH. According to the results, the size of silica nanoparticles was determined at around 100 nm in the coherency of SEM, TEM, and zeta size measurement. The surface modification and success of the antibody immobilization process were also confirmed with EDAX, zeta potential, and FTIR analyses. The amount of anti-transferrin antibody immobilized on the nanoparticles was calculated as 52.64 mg/g silica nanoparticles. The optimum conditions for transferrin recognition were found as 37 °C, 10 min, and 7.4 according to the parameters as temperature, interaction time, and pH, respectively. Meanwhile, the transferrin binding capacity increased from 125.00 mg/g to 686.54 mg/g when the initial concentration increased from 31.25 μg/mL to 250 μg/mL. In the end, the selectivity, in other word interfering effect of competing biomolecules, were confirmed against albumin, gamma-globulin, and ovalbumin. In the light of the results, not only the easy synthesis/modification route but also the efficient detection performance of the silica-based nanoprobe figured out a promising potential.