Akademik Veri Yönetim Sistemi
SCIENTIA IRANICA, cilt.22, sa.3, ss.1330-1336, 2015 (SCI-Expanded)
The aim of the current study is the loading of ceftazidime into the first generation of poly(propyleneimine) dendrimer (PPI-G1) to produce an effective drug delivery system against Pseudomonas aeruginosa. The mechanism of ceftazidime-PPI-G1 dendrimer complex formation is based on interaction between amine groups of dendrimers and carboxylic groups of ceftazidime. PPI-G1 was dissolved in dry tetrahydrofuran (THF), and ceftazidime was added to the solution to prepare the nanodrug. The series of tests, including size, zeta potential, drug release, stability and kinetic evaluation, as well as Scanning Electron Microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy, were performed for characterization of the ceftazidime-loaded PPI-G1. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the nanodrug were determined, with respect to Pseudomonas aeruginosa as the test microorganism. Ceftazidime-PPI-G1 complex was synthesized with the size of 156.6 nm and -10.2 mV zeta potential. The value of loaded ceftazidime was determined to be about 38.46 mol%. A gradual drug release was observed within three days; up to 92% of the loaded ceftazidime. The macrodilution assay demonstrated that PPI-G1 enhances the antibacterial activity of ceftazidime. A new drug delivery system was improved against P. aeruginosa with sustained release and enhanced antibacterial activity. (C) 2015 Sharif University of Technology. All rights reserved.