Liquid chromatographic determination of beta-naphthoxyacetic acid in tomatoes

Gokmen V., Acar J.

JOURNAL OF CHROMATOGRAPHY A, vol.798, pp.167-171, 1998 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 798
  • Publication Date: 1998
  • Doi Number: 10.1016/s0021-9673(97)01192-8
  • Journal Name: JOURNAL OF CHROMATOGRAPHY A
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.167-171
  • Hacettepe University Affiliated: Yes

Abstract

An alternative high-performance liquid chromatographic method for the determination of beta-naphthoxyacetic acid (BNOA) in tomatoes is described. BNOA was extracted from tomatoes with acetone-dichloromethane (2:1). The extract was cleaned up by Bio-Beads S-X3 gel-permeation chromatography and by partitioning. A reversed-phase C-18 column was used for HPLC analysis. The mobile phase was acetonitrile-2% acetic acid in water (50:50, v/v) pumped at a flow-rate of 1.0 ml/min. Retention time of BNOA was ca. 7 min with a percentage coefficient of variation of 0.71. Resolution of BNOA was good on the column. Percentage recoveries of BNOA were 79.5+/-6.82, 94.8+/-2.70 and 86.4+/-16.43 for the corresponding spiking levels of 0.5, 1.0 and 2.0 mu g per g tomato, respectively. Analysis of 10 greenhouse tomato samples from local markets in Ankara showed no BNOA residue. (C) 1998 Elsevier Science B.V.