Human Serum Albumin-Gold Nanoparticle Based Impedimetric Sensor for Sensitive Detection of miRNA-200c

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Akbal Vural Ö., Yaman Y. T., Bolat G., Abacı S.

ELECTROANALYSIS, vol.33, no.4, pp.925-935, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 4
  • Publication Date: 2021
  • Doi Number: 10.1002/elan.202060342
  • Journal Name: ELECTROANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, BIOSIS, Chimica, Communication Abstracts, Metadex, Civil Engineering Abstracts
  • Page Numbers: pp.925-935
  • Keywords: miRNA-200c, human serum albumin&#8722, gold nanoparticle, impedimetric biosensor, FREE ELECTROCHEMICAL DETECTION, MIR-200 FAMILY, MICRORNA, BIOSENSOR, AMPLIFICATION, MECHANISMS, CATALYSIS, GRAPHENE, SURVIVAL, IMPROVE
  • Hacettepe University Affiliated: Yes

Abstract

Here, human serum albumin conjugated gold nanoparticles (HSA-AuNPs) were synthesized by a simple route to develop an impedimetric sensor for miRNA-200c detection based on a selective oligo-hybridization process without any labeling. The synthetic DNA capture probe for miRNA-200c was decorated onto the HSA-AuNPs modified pencil graphite electrodes. Impedimetric signals were monitored after the hybridization process between the DNA probe and target miRNA-200c. HSA-AuNPs adsorption time, incubation time of the capture probe and hybridization time-temperature were optimized. The proposed miRNA-200c biosensor demonstrated proper sensitivity and selectivity, low detection limit (1.13 fM), good reproducibility and simple direct detection of miRNA-200c in serum.