Human Serum Albumin-Gold Nanoparticle Based Impedimetric Sensor for Sensitive Detection of miRNA-200c
ELECTROANALYSIS, cilt.33, sa.4, ss.925-935, 2021 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 33 Sayı: 4
- Basım Tarihi: 2021
- Doi Numarası: 10.1002/elan.202060342
- Dergi Adı: ELECTROANALYSIS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, BIOSIS, Chimica, Communication Abstracts, Metadex, Civil Engineering Abstracts
- Sayfa Sayıları: ss.925-935
- Anahtar Kelimeler: miRNA-200c, human serum albumin−, gold nanoparticle, impedimetric biosensor, FREE ELECTROCHEMICAL DETECTION, MIR-200 FAMILY, MICRORNA, BIOSENSOR, AMPLIFICATION, MECHANISMS, CATALYSIS, GRAPHENE, SURVIVAL, IMPROVE
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Hacettepe Üniversitesi Adresli: Evet
Özet
Here, human serum albumin conjugated gold nanoparticles (HSA-AuNPs) were synthesized by a simple route to develop an impedimetric sensor for miRNA-200c detection based on a selective oligo-hybridization process without any labeling. The synthetic DNA capture probe for miRNA-200c was decorated onto the HSA-AuNPs modified pencil graphite electrodes. Impedimetric signals were monitored after the hybridization process between the DNA probe and target miRNA-200c. HSA-AuNPs adsorption time, incubation time of the capture probe and hybridization time-temperature were optimized. The proposed miRNA-200c biosensor demonstrated proper sensitivity and selectivity, low detection limit (1.13 fM), good reproducibility and simple direct detection of miRNA-200c in serum.