Locally administrated single-dose teriparatide affects critical-size rabbit calvarial defects: A histological, histomorphometric and micro-CT study

Özer T., Başlarlı O., Aktaş A., Barış E., Çelik H. H., Ocak M.

ACTA ORTHOPAEDICA ET TRAUMATOLOGICA TURCICA, cilt.53, ss.478-484, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 53
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.aott.2019.08.007
  • Dergi Adı: ACTA ORTHOPAEDICA ET TRAUMATOLOGICA TURCICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.478-484
  • Anahtar Kelimeler: Animal models, Bone grafting, Bone histomorphometry, Micro-computed tomography, Parathyroid hormone, PARATHYROID-HORMONE 1-34, BONE REGENERATION, CALLUS FORMATION, PTH, AUGMENTATION, TURNOVER, GRAFTS, DRUG, TIME
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Objectives: Teriparatide (PTH 1-34, rhPTH), a synthetic recombinant human protein, consists of the first 34 amino acids of the parathyroid hormone. It is a potent inducer of osteoblastic activity when administered systemically, and its effects on local administration are still not clear. This study aims to evaluate the effects of teriparatide on a rabbit defect model with local xenogen grafts histomorphometrically and radiologically.

Methods: For this purpose, two 10 mm diameter critical-size defects were created in 16 rabbits. In the control group, the defect area was filled with a xenogen graft, while in the teriparatide group (PTH 1-34), a xenogen graft combination with 20 mcg teriparate was used. For both 4 - week and 8 - week study groups, new bone, residual graft, and soft tissue areas were evaluated as well as bone volume histomorphometrically and radiologically.

Results: Histomorphometrically, there was a significant difference in new bone area values at the 8th week (p < 0.05), but there was no significant difference between the 4 - week values (p > 0.05). There was no statistically significant difference between the groups at both 4 and 8 weeks (p > 0.05). In the radiologically measured total bone volume values, PTH1-34 group values were found to be significantly higher for both 4 - and 8 - weeks values compared to the control groups (p < 0.05). 

Conclusion: In this study, rhPTH, which is used locally in defect areas to be repaired with bone grafts, increases both new bone volume and total bone volume.

 

Keywords: animal models, bone grafting, bone histomorphometry, micro-computed tomography, parathyroid hormone