Determination and validation of aprepitant in rat plasma using LC-MS/MS.

Erdogar N., REÇBER T., B Iskit A. B., BİLENSOY E., KIR S., NEMUTLU E.

Bioanalysis, cilt.13, sa.5, ss.363-372, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 5
  • Basım Tarihi: 2021
  • Doi Numarası: 10.4155/bio-2020-0293
  • Dergi Adı: Bioanalysis
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.363-372
  • Anahtar Kelimeler: aprepitant, LC-MS/MS, pharmacokinetics, plasma, validation, PERFORMANCE LIQUID-CHROMATOGRAPHY, CHEMOTHERAPY-INDUCED NAUSEA, P RECEPTOR ANTAGONIST, PHARMACOKINETICS, PREVENTION
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Aim: The assessment of efficacy should be paralleled with extensive pharmacokinetic parameters, and a valid bioanalytical method is a pre-condition for accurate plasma concentration. Materials & methods: A simple, specific, rapid and sensitive LC-MS/MS method has been developed for quantitative analysis of aprepitant in rat plasma. A C18 column was used as stationary phase and the mobile phase consisted of a mixture of formic acid in water and formic acid in acetonitrile. Quantification was performed using multiple reaction monitoring mode. Results: The selectivity, linearity, accuracy, precision, robustness and ruggedness of the method were evaluated in accordance with bioanalytical method validation guideline of ICH and all results were within the acceptable range. Conclusion: The validated LC-MS/MS method was found to be useful for the quantitative analysis of aprepitant in rat plasma samples.