Protective Effect of Rutin on the Ischemia/Reperfusion Induced Damage in Rat Kidney

Korkmaz A., KOLANKAYA D.

JOURNAL OF SURGICAL RESEARCH, vol.164, no.2, pp.309-315, 2010 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 164 Issue: 2
  • Publication Date: 2010
  • Doi Number: 10.1016/j.jss.2009.03.022
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.309-315
  • Hacettepe University Affiliated: Yes


Reactive oxygen species (ROS) are suggested to participate in ischemia/reperfusion (I/R) injury in the kidney This study was designed to mvestigate the effect of rutin, a bioflavonoid, in I/R induced renal injury Wistar albino rats were unilaterally nephrectomized, and 2 wk later they were subjected to 45min of left renal pedicle occlusion followed by 3h of reperfusion Either rutin (1g/kg) or saline was administrated (i p) 1h prior to ischemia At the end of the reperfusion period, kidney samples were taken for determination of renal malondialdehyde (MDA) and glutathione (GSH) levels, manganese-superoxide dismutase (MnSOD) activity and histological examination Serum creatinine, blood urea nitrogen (BUN), and lactate dehydrogenase (LDH) concentrations were measured for the evaluation of renal function I/R caused a significant decrease in GSH level and MnSOD activity, which was accompanied by a significant increase in MBA level of kidney tissues Similarly, serum BUN and creatinine levels, as well as LDH were elevated in the I/R group compared with the control group Pretreatment of rats with rutin (1g/kg/ i p) significantly attenuated renal dysfunction, reduced elevated MBA levels, and restored the depleted MnSOD activity and GSH levels These beneficial changes m the biochemical parameters were also associated with parallel changes in histopathological appearance These findings suggest that ROS play a causal role in I/R induced renal injury, and that rutin exerts renal protective effects, probably by inhibiting ROS and antioxidant activities (C) 2010 Elsevier Inc All rights reserved.