In this paper, chromosome number, karyotype and pollen morphology of Centaurea goeksunensis Aytac & H. Duman, described in 2005, are studied for the first time. Centaurea goeksunensis is found to be tetraploid (2n=4x=60). This is the second record of polyploidy in Centaurea sect. Psephelloideae (Boiss.) Sons. The basic chromosome number of C. goeksunensis was found to be x=15 and the haploid karyotype formula is 18 m+10 sm+2 st. The pollen grains of C. goeksunensis were found to be radially symmetric, isopolar and tricolporate. They are intersemiangular in polar view and prolate-sphaeroidal in equatorial view. The exine is thickened at the poles.