Polyethylenimine attached-poly(3-chloro-2-hydroxypropyl methacrylate-co-ethylene dimethacrylate) monosized-porous microspheres as a new separation medium for polar compounds
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, vol.441, pp.629-637, 2014 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 441
- Publication Date: 2014
- Doi Number: 10.1016/j.colsurfa.2013.10.030
- Journal Name: COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.629-637
- Hacettepe University Affiliated: Yes
Abstract
Monosized-porous and hydrophilic polymer microspheres with reactive chlorine functionality were first obtained in the form of poly(3-chloro-2-hydroxypropyl methacrylate-co-ethylene dimethacrylate), poly(HPMA-Cl-co-EDMA) copolymer by a new seeded polymerization. A macromolecular ligand, polyethylenimine (PEI) was covalently attached onto the poly(HPMA-Cl-co-EDMA) microspheres via a single stage reaction between 3-chloro-2-hydroxypropyl and amine groups. PEI attached-poly(HPMA-Cl-co-EDMA) microspheres were slurry packed into the microbore columns and used as stationary phase in hydrophilic interaction chromatography (HILIC) mode. The separation of various compounds, nucleosides, polar organics and peptides was successfully performed. The results showed that hydrophilic poly(HPMA-Cl-co-EDMA) microspheres are a new, alternative starting material for the synthesis of stationary media with different functionalities for various liquid chromatographic applications. (C) 2013 Elsevier B.V. All rights reserved.