Microfluidic Production of Antibacterial Alginate (Alg-PEI/AgNP) Microbubble-Templated Scaffolds for Biomedical Applications: Controlled Pore Architecture and Sustained Silver Release
Macromolecular Materials and Engineering, cilt.311, sa.8, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 311 Sayı: 8
- Basım Tarihi: 2026
- Doi Numarası: 10.1002/mame.70327
- Dergi Adı: Macromolecular Materials and Engineering
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Applied Science & Technology Source, Chemical Abstracts Core, Chimica, Compendex, INSPEC, Directory of Open Access Journals, Academic Search Ultimate (EBSCO), Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Engineering Source (EBSCO), Materials Science & Engineering Collection (ProQuest), Technology Collection (ProQuest)
- Anahtar Kelimeler: alginate porous structure, antibacterial, microbubble, microfluidic, silver nanoparticle, T-junction
- Hacettepe Üniversitesi Adresli: Evet
Özet
Microfluidic-derived microbubble-templated scaffolds (Mt-Ss) enable controllable and homogeneous pore architectures in tissue constructs, offering advantages over conventional fabrication methods. In this study, we developed antibacterial alginate-polyethyleneimine (Alg-PEI) scaffolds incorporating silver nanoparticles (AgNPs) using a T-junction microfluidic system to construct well-ordered Mt-Ss for potential wound-healing applications. AgNPs were synthesized with an average diameter of 25.63 ± 3.7 nm, a polydispersity index (PDI) of 0.11, and a zeta potential of −21.7 ± 9.1 mV, indicating near indicating narrow size distribution approaching monodispersity and electrosterically stabilized nanoparticle formation. The resulting Mt-Ss exhibited tunable pore sizes ranging from 90 to 175 µm, depending on PEI and AgNP concentrations, and exceptional water retention capacities of 3685–4300%, which are favorable for managing exudative wounds. ICP-MS analysis revealed a sustained total silver release profile up to 168 h, with the Alg-PEI(0.1)/AgNP0.1 formulation demonstrating the highest cumulative release of 8.7 ppm. Notably, Mt-Ss containing 1% PEI and 0.05% AgNPs exhibited cytocompatibility and antibacterial activity against Gram-negative Escherichia coli (E. coli-ATCC 25922), Gram-positive Staphylococcus aureus (S. aureus-ATCC 29213), and clinically relevant Methicillin-resistant S. aureus (MRSA, ATCC 43300). Overall, Alg-PEI/AgNP Mt-Ss demonstrate sustained antibacterial efficacy, superior fluid absorption, and favorable in vitro cytocompatibility, representing a potential candidate for the future management of infected wound environments.