Human Papillomavirus Types in Invasive Cervical Cancer Specimens From Turkey

USUBÜTÜN A., Alemany L., KÜÇÜKALİ T., AYHAN A., YÜCE K., de Sanjose S., ...More

INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY, vol.28, no.6, pp.541-548, 2009 (SCI-Expanded) identifier identifier identifier


The main aim of the study is to describe the human papillomavirus (HPV) type-specific distribution in invasive cervical cancer (ICC) specimens from Turkey Paraffin-embedded ICC specimens were identified from the histopathologic archives of the Hacettepe University Medical School in Turkey HPV detection was carried out through amplification of HPV DNA by a SPF-10 broad-spectrum primer polymerase chain reaction and subsequently followed by DNA enzyme immunoassay and genotyping by LiPA25 (version 1). Two hundred seventy-seven ICC cases diagnosed between 1993 and 2004 were retrieved. After histologic evaluation and human beta-globin gene analysis for sample quality, 248 cases were considered suitable for HPV/DNA testing HPV prevalence was 93.5% (232/248, 95% confidence interval 90.5%-96.6%). The five most common HPV types identified as single types among HPV-positive cases were HPV16 (64.7%), HPV18 (9.9%), HPV45 (9.9%), HPV31 (3.0%), and HPV33 (2.2%) The study shows that in Turkey; HPV16/HPV18 accounted for 75.4% (95% confidence interval 69.9%-81.0%) of HPV-positive ICC cases. This information is essential to evaluate the potential impact of the HPV vaccines in the country