A continuous fixed bed study was carried out by using dried Rhizopus arrhizus as a biosorbent for the removal of three reactive dyes; Gemacion (Procion) Red H-E7B (GR), a monoclorotriazine mono-azo type reactive dye; Gemazol Turquise Blue-G (GTB), a vinyl sulfone mono-azo type reactive dye and Gernactive (Reactive) Black HFGR (GB), a vinyl sulfone di-azo type reactive dye from aqueous solution. The effect of operating parameters such as flow rate and inlet dye concentration on the sorption characteristics of R. arrhizus was investigated at pH 2.0 and at 25 degrees C for each dye. Data confirmed that the total amount of sorbed dye decreased with increasing flow rate and increased with increasing inlet dye concentration for each dye. The column biosorption capacity of dried R. arrhizus was 1007.8 mg g(-1) for GR dye, 823.8 mg g(-1) for GTB dye and 635.7 mg g-1 for GB dye at the highest inlet dye concentration of approximately 750 mg 1(-1) and at the minimum flow rate of 0.8 ml min(-1). Thomas and Yoon-Nelson models were applied to experimental data to predict the breakthrough curves and to determine the biosorption capacity of the column for each dye useful for process design. Both models were found suitable for describing the whole dynamic behavior of the column with respect to flow rate and inlet dye concentration. (C) 2006 Elsevier B.V. All rights reserved.