Akademik Veri Yönetim Sistemi
"Türk Fizyolojik Bilimler Derneği 45. Ulusal Fizyoloji Kongresi", Aydın, Türkiye, 31 Ekim - 03 Kasım 2019, ss.34
It is known that oxidative stress
is the main factor in the formation of disuse muscle atrophy, the most common
type of muscle atrophy. The first important damage which is exerted by
oxidative stress on proteases such as calpain-1 has been demonstrated in
several studies. Treatment options include antioxidants, and in these groups
mitochondrial targeting antioxidants such as melatonin have been shown to be
more effective.
AIM: In this study, we aimed to
determine the effect of melatonin treatment on oxidative stress-induced atrophy
morphological change, calpain-1 expression and redox balance in C2C12 muscle
cells.
METHOD: We have used H2O2 to
generate oxidative stress. Four groups of C2C12 cells were created as
K(Control), M(Melatonin), H(H2O2 ), M+H (Melatonin+ H2O2 ) groups. For
morphological evaluation, measurement of myotube diameter with Image J Program
was performed. In the second step, the amount of calpain-1 protein from total
protein lysates was measured by western blot method. TAS and TOS kits were used
to find out the antioxidant/oxidant statuses of the cells. Statistical analyzes
were performed in SPSS program. The level of significance was set at p
<0.05.
RESULTS: There was a
statistically significant decrease in the mean myotube diameters of the H group
compared to the other groups (K, M, M+H) and statistically significant increase
in mean myotube diameter of M group compared to K and H groups. The expression
level of calpain-1 protein was increased in H, M and M+H groups compared with K
group. TOS was higher in the H group than other groups. The highest mean TAS
value was seen in the H group (rebound effect).
CONCLUSION: These results suggest
that H2O2 produces muscle atrophy in C2C12 cells and that melatonin prevents morphological
change and in addition to calpain-1 protein, other mechanisms can also take part
in the prevention of disuse muscle atrophy. Functional studies need to be
carried out to better explain this effect.