Treatment of tumour tissue with radio-frequency hyperthermia (using antibody-carrying nanoparticles)

Didarian R., VARGEL İ.

IET NANOBIOTECHNOLOGY, cilt.15, sa.8, ss.639-653, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 8
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1049/nbt2.12061
  • Dergi Adı: IET NANOBIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, Business Source Elite, Business Source Premier, Communication Abstracts, Compendex, Computer & Applied Sciences, EMBASE, INSPEC, MEDLINE, Metadex, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.639-653
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Intelligent inorganic nanoparticles were designed and produced for use in imaging and annihilating tumour cells by radio-frequency (RF) hyperthermia. Nanoparticles synthesised to provide RF hyperthermia must have magnetite properties. For this purpose, magnetite nanoparticles were first synthesised by the coprecipitation method (10-15 NM). These superparamagnetic nanoparticles were then covered with gold ions without losing their magnetic properties. In this step, gold ions are reduced around the magnetite nanoparticles. Surface modification of the gold-coated magnetic nanoparticles was performed in the next step. A self-assembled monolayer was created using cysteamine (2-aminoethanethiol) molecules, which have two different end groups (SH and NH2). These molecules react with the gold surface by SH groups. The NH2 groups give a positive charge to the nanoparticles. After that, a monoclonal antibody (Monoclonal Anti-N-CAM Clone NCAM-OB11) was immobilised by the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide method. Then, the antenna RF system (144.00015 MHz) was created for RF hyperthermia. The antibody-nanoparticle binding rate and cytotoxicity tests were followed by in vitro and in vivo experiments. As the main result, antibody-bound gold-coated magnetic nanoparticles were successfully connected to tumour cells. After RF hyperthermia, the tumour size decreased owing to apoptosis and necrosis of tumour cells.