An easy two-step purification method for human leucocyte myeloperoxidase

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Atıf İçin Kopyala

Sarkarati B., KARAAĞAÇ AKYOL T., Kilinc K.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, cilt.40, sa.5, ss.410-416, 2015 (SCI-Expanded)

• Yayın Türü: Makale / Tam Makale
• Cilt numarası: 40 Sayı: 5
• Basım Tarihi: 2015
• Doi Numarası: 10.1515/tjb-2015-0035
• Dergi Adı: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
• Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
• Sayfa Sayıları: ss.410-416
• Hacettepe Üniversitesi Adresli: Evet

Özet

Objective: The object of this study is to describe a simple, rapid and cost effective method for purification of human leucocyte myeloperoxidase from a single donor. Myelopeoxidase (MPO) was purified by a two step procedure consisting of concanavalin-A Sepharose 4B affinity chromatography followed by CM-Sephadex cation exchange chromatography.