Development, characterization and research of efficacy on in vitro cell culture of glucosamine carrying hyaluronic acid nanoparticles

Şahin Ş., BİLGİÇ E., Salimi K., Tuncel A., KARAOSMANOĞLU B., Taşkıran E. Z., ...More

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, vol.52, pp.393-402, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 52
  • Publication Date: 2019
  • Doi Number: 10.1016/j.jddst.2019.05.007
  • Journal Name: JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.393-402
  • Keywords: Osteoarthritis, cartilage, chondrocyte, hyaluronic acid, glucosamine, nanoparticle, KNEE OSTEOARTHRITIS, CHITOSAN NANOPARTICLES, CHONDROITIN SULFATE, ARTICULAR-CARTILAGE, CHONDROCYTES, EXPRESSION, PROTEOGLYCAN, MORTALITY, MODEL
  • Hacettepe University Affiliated: Yes

Abstract

Intra-articular hyaluronic acid (HA) and oral glucosamine (GA) are frequently used to decrease pain and increase function in osteoarthritis patients. Injectable chondroitin sulfate and HA improve effectiveness however a formulation that combines GA and HA is currently not available. Aims of this study were to synthesize and characterize N-acetyl-d-GA immobilized HA based nanoparticles [GA-PEG@nano(HA)] to increase extracellular matrix production and to decrease OA markers of healthy and OA chondrocytes. Mean size of synthesized GA-PEG@nano(HA) was 175 nm and 20% of GA released from these composites during 21 days. Released GA reduced the proliferation of chondrosarcoma cells. Chondrogenic marker expression of bone marrow mesenchymal stem cells decreased slightly but not significantly. osteoarthritis and extracellular matrix markers of healthy and osteoarthritic chondrocytes at seven days in culture remained unchanged. In conclusion, GA-PEG@ nano(HA) composites had limited effects on mesenchymal stem cells, healthy and osteoarthritic chondrocytes.