The frequency of PER-1 type extended spectrum beta-lactamases in nosocomial isolates-of Pseudomonas aeruginosa

ZARAKOLU P., METAN G., AYDİN N. G., ALTUN B., HASÇELİK A. G., AKOVA M.

MIKROBIYOLOJI BULTENI, cilt.41, sa.3, ss.363-367, 2007 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 3
  • Basım Tarihi: 2007
  • Dergi Adı: MIKROBIYOLOJI BULTENI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.363-367
  • Hacettepe Üniversitesi Adresli: Evet

Özet

The aim of this study was to determine the frequency of PER-1 type extended-spectrum beta-lactamase (ESBL) in nosocomial Pseudomonas aeruginosa strains isolated in Hacettepe University Adult Hospital. Sixty-seven non-duplicate P.aeruginosa isolates from patients with nosocomial infections between January 2002 and December 2004 were included in the study. The isolates were identified at species-level by Sceptor (Becton Dickinson, USA) system, and all the strains were stored at -80 degrees C until further testing. Polymerase chain reaction (PCR) was performed for the detection of bla(PER-1) genes, and PFGE analysis was used to investigate their genetic relatedness. The antimicrobial susceptibilities of the PER-1 positive isolates were determined by Etest (AB Biodisk, Solna, Sweden) method. According to the results of PCR, 22.7% (15/67) of the isolates were positive for PER-1 enzyme. Those 15 bla(PER-1) positive isolates showed eight different PFGE patterns, indicating the presence of multiple clones. Of the PER-1 positive P.aeruginosa isolates, nine were resistant to imipenem/meropenem, and 11 were resistant to piperacillin-tazobactam and tobramycin. The epidemiological investigation of multidrug resistant P.aeruginosa should give important clues for the initial empirical therapy, especially in certain geographic locations where ESBL-producing P.aeruginosa strains seemed to be highly prevalent.