The frequency of PER-1 type extended spectrum beta-lactamases in nosocomial isolates-of Pseudomonas aeruginosa


ZARAKOLU P., METAN G., AYDİN N. G. , ALTUN B., HASÇELİK A. G. , AKOVA M.

MIKROBIYOLOJI BULTENI, vol.41, no.3, pp.363-367, 2007 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 3
  • Publication Date: 2007
  • Journal Name: MIKROBIYOLOJI BULTENI
  • Journal Indexes: Science Citation Index Expanded, Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.363-367

Abstract

The aim of this study was to determine the frequency of PER-1 type extended-spectrum beta-lactamase (ESBL) in nosocomial Pseudomonas aeruginosa strains isolated in Hacettepe University Adult Hospital. Sixty-seven non-duplicate P.aeruginosa isolates from patients with nosocomial infections between January 2002 and December 2004 were included in the study. The isolates were identified at species-level by Sceptor (Becton Dickinson, USA) system, and all the strains were stored at -80 degrees C until further testing. Polymerase chain reaction (PCR) was performed for the detection of bla(PER-1) genes, and PFGE analysis was used to investigate their genetic relatedness. The antimicrobial susceptibilities of the PER-1 positive isolates were determined by Etest (AB Biodisk, Solna, Sweden) method. According to the results of PCR, 22.7% (15/67) of the isolates were positive for PER-1 enzyme. Those 15 bla(PER-1) positive isolates showed eight different PFGE patterns, indicating the presence of multiple clones. Of the PER-1 positive P.aeruginosa isolates, nine were resistant to imipenem/meropenem, and 11 were resistant to piperacillin-tazobactam and tobramycin. The epidemiological investigation of multidrug resistant P.aeruginosa should give important clues for the initial empirical therapy, especially in certain geographic locations where ESBL-producing P.aeruginosa strains seemed to be highly prevalent.