Investigation of BRAF mutation analysis with different technical platforms in metastatic melanoma

Sener E., Yildirim P., Tan A., GÖKÖZ Ö., Tezel G. G.

PATHOLOGY RESEARCH AND PRACTICE, cilt.213, sa.5, ss.522-530, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 213 Sayı: 5
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.prp.2017.01.010
  • Dergi Adı: PATHOLOGY RESEARCH AND PRACTICE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.522-530
  • Hacettepe Üniversitesi Adresli: Evet

Özet

In metastatic melanoma, the detection of somatic mutations in the BRAF gene is crucial regarding patient selection for targeted therapy. Several screening methods have been developed to identify BRAF gene mutations. In this study, our objective was to evaluate the detection of the BRAF V600 mutations using two molecular methods, real-time polymerase chain (real-time PCR) assay and pyrosequencing, and immunohistochemistry (IHC), and to compare the results of these different technical platforms.