The study area is located in the southern part of the Central Anatolia Neogene Basin (north of Karapinar Town and Aci Gol, Konya). The Insuyu Limestone Unit, which has a total thickness of 100-400 m, is composed of claystone, sandstone, conglomerate and chert. There are also andezite and pyroclastic interclations within Insuyu limestone. The claystone layers of this unit are white to pink in color and contain abundant Ostracoda and a few fresh water Gastropoda. In this study, 160 samples from 8 stratigraphic sections were collected but only samples having limestone lithology contained ostracods. A total of 16 ostracods taxa (11 previously known and five belonging to open nomenclature) are systematically described as Cyprideis pannonica, C torosa, Ilyocypris gibba, I. bradyi, Candona neglecta, C. candida, C. altoides, C. decimai, Pseudocandona compressa, Heterocypris salina, Zonocypris membranea, Ilyocypris sp. Candona sp.1, Candona sp.2, Pseudocandona sp. and Cypridopsis sp. Based on this Ostracoda fauna, the age of this unit is assigned as Pliocene-Early Pleistocene. This fauna indicates a few brackish but dominantly freshwater conditions in a lacustrine environment.