Integrative proteomics and metabolomics approach to elucidate the antimicrobial effect of simvastatin on Escherichia coli

Kocak E., NEMUTLU E., KIR S., SAĞIROĞLU M., ÖZKUL KOÇAK C.

BIOMEDICAL CHROMATOGRAPHY, cilt.35, sa.10, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 35 Sayı: 10
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1002/bmc.5180
  • Dergi Adı: BIOMEDICAL CHROMATOGRAPHY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: antimicrobial activity, Escherichia coli, metabolomics, proteomics, simvastatin, IN-VIVO, STATIN THERAPY, EFFLUX PUMP, ANTIBIOTICS, RESISTANCE, REVEALS, TARGET, VIRULENCE, VITRO, TOLC
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Globally, simvastatin is one of the most commonly used statin drugs. Its antimicrobial properties have been investigated against various pathogens. However, its effect on biological processes in bacteria has been unclear. This study focused on altered biological and metabolic processes at protein and metabolite levels induced by simvastatin. MS-based proteomics and metabolomics were used to investigate the altered proteins and metabolites between experimental groups. Proteomics results showed that simvastatin induced various antimicrobial targets such as chaperon protein DnaK and cell division protein FtsZ. Metabolomics results revealed phenotypic changes in cells under simvastatin stress. Integrated proteomics and metabolomics result indicated that various metabolic processes were altered to adapt to stress conditions. Energy metabolism (glycolysis, tricarboxylic acid cycle, etc.), amino acid synthesis and ribosomal proteins, and purine and pyrimidine synthesis were induced by the effect of simvastatin. This study will contribute to the understanding of antimicrobial properties of statin drugs.