Effects of different substrates on growth and glycerol production kinetics of a wine yeast strain Saccharomyces cerevisiae Narince 3


Yalcin S., Ozbas Z. Y.

PROCESS BIOCHEMISTRY, vol.39, no.10, pp.1285-1291, 2004 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 10
  • Publication Date: 2004
  • Doi Number: 10.1016/s0032-9592(03)00252-8
  • Title of Journal : PROCESS BIOCHEMISTRY
  • Page Numbers: pp.1285-1291
  • Keywords: glycerol production, Saccharomyces cerevisiae, fermentation, osmophilic yeast, growth kinetics, wine, FERMENTATION

Abstract

Glycerol, is important industrial product that can be produced using osmophilic yeasts. A study was carried out to determine the effects of glucose, fructose, and sucrose on growth and glycerol production kinetics of a wine yeast strain Saccharomyces cerevisiae Narince 3 in a batch system. Depending on the determined values of Monod constants, sucrose (K-s = 44.53 g/l) was the most suitable substrate for yeast growth. Initial glucose, fructose and sucrose concentrations necessary for maximum specific yeast growth rate were 125, 175, and 200 g/l, respectively. The glycerol production rate was highest in fructose medium and reached a maximum level in the medium of 350 g/l of initial fructose concentration. In glucose medium, optimal glycerol production was observed at 300 g/l initial glucose concentration and at 400 g/l initial sucrose concentration in medium containing sucrose. In grape juice medium, maximum glycerol concentration and dry weight were 14.1 and 8.0 g/l, respectively. (C) 2003 Elsevier Ltd. All rights reserved.