Label-Free impedimetric miRNA-192 genosensor platform using graphene oxide decorated peptide nanotubes composite

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Bolat G., Akbal Vural Ö., Yaman Y. T., Abacı S.

MICROCHEMICAL JOURNAL, cilt.106218, ss.106218, 2021 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 106218
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.microc.2021.106218
  • Dergi Adı: MICROCHEMICAL JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Food Science & Technology Abstracts, Index Islamicus, Veterinary Science Database
  • Sayfa Sayıları: ss.106218
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Here, we report on the preparation of a sensor platform that implies diphenylalanine based self-assembled peptide nanotubes (PNTs) with graphene oxide (GO) on graphite electrodes and its evaluation for quantification of diabetic nephropathy and cancer-related microRNA-192. The PNT-GO structures were characterized and used to functionalize pencil graphite electrodes (PGE) for biosensor construction. Measured impedance was utilized as the signal to evaluate the hybridization between miRNA-192 and its corresponding ss-DNA probe owing to the features of PNT-GO, for the first time. PNT-GO/PGE showed high sensitivity for electrochemical miRNA-192 analysis with a very wide dynamic detection range of 10 fM – 1.0 nM and a low sensing limit of 8 fM. The evaluation of the developed impedimetric biosensor in peripheral serum samples revealed its potential for future clinical applications and diverse biomedical applications without the aid for extraction, sample pretreatment or amplification steps.