Characterization of serum extracellular vesicles and their differential level of miR-197-3p in familial Mediterranean fever patients

Tavukcuoglu Z., AKKAYA ULUM Z. Y., YERSAL N., Horzum U., AKBABA T. H., KARADAĞ Ö., ...Daha Fazla

Nucleosides, Nucleotides and Nucleic Acids, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1080/15257770.2023.2283187
  • Dergi Adı: Nucleosides, Nucleotides and Nucleic Acids
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Chemical Abstracts Core, Chimica, MEDLINE
  • Anahtar Kelimeler: extracellular vesicles, Familial Mediterranean fever, flow cytometry, miRNAs, transmission electron microscopy
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Objectives: The aim of this study was to analyze the existence of miRNAs derived from serum extracellular vesicles (EVs) in familial Mediterranean fever (FMF) patients. Our group has previously shown the association of certain miRNAs with FMF. Methods: Serum samples of adult and pediatric FMF patients and their age matched controls were used in the study. Serum EVs were characterized by transmission electron microscopy (TEM) and flow cytometry. RNAs were isolated from EVs and levels of miR-197-3p and miR-20a-5p were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Results: EV characterization using TEM demonstrated fraction of 30–120 nm-sized particles with cup-shaped morphology. Flow cytometry results revealed the CD63 and CD81 positive populations as 53.3% in serum EVs. We showed that miR-197-3p and miR-20a-5p were “circulating miRNAs” and carried in EVs of FMF patients and controls. In FMF patients, level of miR-197-3p was significantly decreased. There was no significant alteration in the level for miR-20a-5p between patients and controls. Conclusion: We showed the differential level of miR-197-3p in serum EVs of the FMF patients. miR-197-3p’s potential as a biomarker and therapeutic target in FMF pathogenesis warrants further investigation.