Macroporous monolithic gels, cryogels, with immobilized phages from phage-display library as a new platform for fast development of affinity adsorbent capable of target capture from crude feeds


Noppe W., Plieva F. M. , Vanhoorelbeke K., Deckmyn H., Tuncel M., Tuncel A. , ...More

JOURNAL OF BIOTECHNOLOGY, vol.131, no.3, pp.293-299, 2007 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 131 Issue: 3
  • Publication Date: 2007
  • Doi Number: 10.1016/j.jbiotec.2007.06.021
  • Title of Journal : JOURNAL OF BIOTECHNOLOGY
  • Page Numbers: pp.293-299

Abstract

Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10-100 mu m) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 mu m) phage particles as ligands without any risk of blocking the monolithic column. The macroporous monolithic columns were successfully used for the direct affinity capture of target proteins from particulate containing feeds like milk containing casein micelles and fat globules (1-10 mu m in size) or even whole blood containing blood cells (up to 20 mu m in size). The newly developed platform based on selected bacteriophages immobilized within macropores of the monolithic cryogels presents a convenient alternative to antibodies for fast and selective development of the specific adsorbent. (C) 2007 Elsevier B.V. All rights reserved.