Critical steps in electronic volume quantification of gingival crevicular fluid: the potential impact of evaporation, fluid retention, local conditions and repeated measurements


Tozum T. F. , Hatipoglu H., Yamalik N. , Gursel M., Alptekin N., Ataoglu T., ...More

JOURNAL OF PERIODONTAL RESEARCH, vol.39, no.5, pp.344-357, 2004 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 5
  • Publication Date: 2004
  • Doi Number: 10.1111/j.1600-0765.2004.00758.x
  • Title of Journal : JOURNAL OF PERIODONTAL RESEARCH
  • Page Numbers: pp.344-357

Abstract

Background and objectives: Various methodological factors may operate during clinical gingival crevicular fluid (GCF) sampling, volume quantification or subsequent laboratory analysis. For precise volume quantification, specific concern for generation and maintenance of a reliable calibration curve, the potential risk of GCF loss as a result of evaporation or fluid retention on actual volume and the impact of local conditions is needed because each of these factors may act as a source of subsequent volumetric distortions. Thus, the present study aimed to analyse the impact of sample transfer time on the rate of evaporation and the possibility of fluid retention, and the impact of local conditions and number of replicated measurements on the reliability of calibration data.