Affinity purification lipase from wheat germ: comparison of hydrophobic and metal chelation effect


Kose K., Erol K., Kose D. A. , Evci E., UZUN L.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, vol.45, no.3, pp.574-583, 2017 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 45 Issue: 3
  • Publication Date: 2017
  • Doi Number: 10.3109/21691401.2016.1161642
  • Title of Journal : ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Page Numbers: pp.574-583

Abstract

Cryogels are used quite a lot nowadays for adsorption studies as synthetic adsorbents. In this study, lipase enzyme (obtained from Candida cylindracea) adsorption capacity of poly(2-hydroxyethyl methacrylate- N-methacryloyl-L-tryptophan), poly(HEMA-MATrp), and Cu(II) ions immobilized poly(HEMA-MATrp), poly(HEMA-MATrp)-Cu(II), cryogel membranes were synthesized to determine and compare the adsorption behavior of lipase enzyme. In this regard, the effect of pH, interaction time, lipase initial concentration, temperature and ionic strength on the adsorption capacity of these membranes was investigated. Maximum lipase enzyme adsorption capacities of poly(HEMA-MATrp) and poly(HEMA-MATrp)-Cu(II) cryogel membranes were determined as 166.4mg/g and 196.4mg/g, respectively.