Affinity purification lipase from wheat germ: comparison of hydrophobic and metal chelation effect

Kose, Kazim; Erol, Kadir; Kose, Dursun; Evci, Emre; UZUN, LOKMAN

doi:10.3109/21691401.2016.1161642

Affinity purification lipase from wheat germ: comparison of hydrophobic and metal chelation effect

Atıf İçin Kopyala

Kose K., Erol K., Kose D. A., Evci E., UZUN L.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, cilt.45, sa.3, ss.574-583, 2017 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 45 Sayı: 3
Basım Tarihi: 2017
Doi Numarası: 10.3109/21691401.2016.1161642
Dergi Adı: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.574-583
Hacettepe Üniversitesi Adresli: Evet

Özet

Cryogels are used quite a lot nowadays for adsorption studies as synthetic adsorbents. In this study, lipase enzyme (obtained from Candida cylindracea) adsorption capacity of poly(2-hydroxyethyl methacrylate- N-methacryloyl-L-tryptophan), poly(HEMA-MATrp), and Cu(II) ions immobilized poly(HEMA-MATrp), poly(HEMA-MATrp)-Cu(II), cryogel membranes were synthesized to determine and compare the adsorption behavior of lipase enzyme. In this regard, the effect of pH, interaction time, lipase initial concentration, temperature and ionic strength on the adsorption capacity of these membranes was investigated. Maximum lipase enzyme adsorption capacities of poly(HEMA-MATrp) and poly(HEMA-MATrp)-Cu(II) cryogel membranes were determined as 166.4mg/g and 196.4mg/g, respectively.