Eco-friendly Production of Bacterial Cellulose with Komagataeibacter intermedius Strain by Using Jasminum sambac and Camellia sinensis Plants


Journal of Polymers and the Environment, vol.32, no.1, pp.460-477, 2024 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 1
  • Publication Date: 2024
  • Doi Number: 10.1007/s10924-023-03081-9
  • Journal Name: Journal of Polymers and the Environment
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Aerospace Database, Agricultural & Environmental Science Database, BIOSIS, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, Environment Index, Geobase, Greenfile, Metadex, Pollution Abstracts, Civil Engineering Abstracts
  • Page Numbers: pp.460-477
  • Keywords: Bacterial cellulose, Camellia sinensis, Jasminum sambac, Komagataiebacter intermedius, Physicochemical characterization
  • Hacettepe University Affiliated: Yes


The widespread use of bacterial cellulose (BC) in industrial processes increases the demand for this valuable biomaterial. Accordingly, research mainly focuses on investigating novel substrates for BC production with low cost and high productivity. In this respect, the optimization and physicochemical characterization of BC membranes by using Jasminum sambac and Camellia sinensis as low-cost substrates with Komagataeibacter intermedius strain were examined. BC production increased with these plants under optimized conditions: 20 g/100 mL J. sambac, 8% glucose, 10% inoculum and 30 g/100 mL C. sinensis, 8% fructose, 20% inoculum at pH = 6.0 for 7 days statically with yields of 12.16 and 14.58 g/L, respectively. In comparison with BC obtained from HS (5.59 g/L), the BC yield increased 2.17 and 2.61 times by using J. sambac and C. sinensis, respectively. Additionally, 40.71 and 37.87% of the total phenolics (31.49 and 43.67 mg/g), 14.17 and 48.4% of the total proteins (13.4 and 33 mg/g) and 73.57 and 79.85% of the reducing sugars (1.4 and 2.78 g/L) in J. sambac and C. sinensis, respectively were used. J. sambac and C. sinensis plant wastes can be used at least two and nine more times in BC production with a volumetric yield of 1.91 and 7.19 g/L, respectively. BC obtained from C. sinensis plant-based medium had the thinnest fibril size (0.10 − 0.07 μm), exhibited high water holding capacity (106.44%) when compared with BC obtained from HS (98%) and J. sambac plant-based medium (95.85%). Additionally, whereas the cost of BC obtained from HS broth was found to be 1.003 Euro/per g, the cost of BC significantly decreased by 0.27 and 0.39 Euro/g with J. sambac and C. sinensis plant-based media, respectively. BC membranes showed a nanoporous structure and depended on cellulose type 1. The results suggested J. sambac and C. sinensis plants as novel cost-effective substrates for BC production with enhanced volumetric yield, high water holding capacity and excellent physicochemical properties to be used in industrial applications and biotechnological research. Graphical Abstract: [Figure not available: see fulltext.]