Purpose: This study was planned to compare the results of gait training done by conventional methods and Biodex Gait Trainer 2 (TM) in the restoration of gait which is the most important functional loss in unilateral transtibial amputees. Material and methods: The study group consisted of 40 amputees between 20-45 years of age. The subjects were divided into two groups. Amputees who received conventional gait training formed the control group while the study group consisted of amputees who practiced gait with Biodex Gait Trainer 2 (TM). After the patients were assessed for their demographic characteristics, they received gait training for 10 days and they were evaluated by gait trainer for their gait characteristics ( Velocity, stride length, cadence, and step cycle), ambulation index and patient's physiological cost index (PCI) was also assessed. The data was evaluated by suitable statistical methods. Results: No demographic differences was found between the two groups (p>0.05). There were significant differences in favor of the Biodex Gait Trainer 2 (TM) group in gait velocity, gait distance and PCI (p<0.05). Conclusion: Although both forms of gait training was found to be very effective in improving gait characteristics, balance and coordination; gait trainer was found to be more effective in diminishing the energy consumption due to the selection of gait velocity according to personal characteristics.