A comparative study on citric acid production kinetics of two Yarrowia lipolytica strains in two different media

Yalcin S. K., BOZDEMİR M. T., ÖZBAŞ Z. Y.

INDIAN JOURNAL OF BIOTECHNOLOGY, cilt.8, sa.4, ss.408-417, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 8 Sayı: 4
  • Basım Tarihi: 2009
  • Dergi Adı: INDIAN JOURNAL OF BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.408-417
  • Anahtar Kelimeler: Batch fermentation, citric acid, growth kinetics, production kinetics, Yarrowia lipolytica, yeast, MEDIUM CONSTITUENTS, OPTIMIZATION, NITROGEN, YIELD
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Microbial production of citric acid was performed by Yarrowia lipolytica NBRC 1658 and a domestic strain in the media containing glucose or fructose as substrates. The study was carried out in a batch system. The non-competitive substrate inhibition model was proposed for growth of the yeasts in both substrate media. Higher dry mass and lower K, values were obtained in glucose media when compared to fructose media. The highest citric acid concentration (65.1 g/L) was obtained with the domestic strain in the medium containing 200 g/L fructose. In the media containing fructose, maximum citric acid concentration and productivity values determined with the domestic strain were aproximately two-fold greater than those obtained with NBRC 1658 strain. The required initial concentration of glucose or fructose at which best citric acid production properties were observed changed between 100-200 g/L for both of the strains. The ratio of citric acid to isocitric acid was found to change between 11.70-16.62.