Impaired antioxidant enzyme functions with increased lipid peroxidation in epithelial ovarian cancer


ÇAĞLAYAN A. , Katlan D. C. , TUNCER Z. S. , YÜCE K. , SAYAL H. B. , SALMAN M. C. , ...Daha Fazla

IUBMB LIFE, cilt.69, sa.10, ss.802-813, 2017 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 69 Konu: 10
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1002/iub.1675
  • Dergi Adı: IUBMB LIFE
  • Sayfa Sayıları: ss.802-813

Özet

We aimed to identify the possible role of oxidant-antioxidant status in epithelial ovarian cancer (EOC) by measuring (a) antioxidant enzyme (AOE) activities [total superoxide dismutase (SODtotal), manganese-SOD (Mn-SOD), copper,zinc-SOD (Cu,Zn-SOD), catalase (CAT) and glutathione peroxidase (GPx1)], (b) Mn-SOD protein expression, (c) lipid peroxidation markers [malondialdehyde (MDA), 8-epi-prostaglandin-F2 (8-epi-PGF2)] and by evaluating the possible correlations between tumor biomarkers, reproductive hormone levels and all measured parameters, comprehensively. The data obtained from the patients with EOC (M, n=26) evaluated according to the histopathological/clinical characteristics of tumors and compared with data of healthy controls [C-tissue (C1) and C-blood/urine (C2), n=30, respectively). Significantly, low activities of tumor SODtotal (52%), Mn-SOD (42%), Cu,Zn-SOD (55%); high activities of tumor and erythrocyte CAT (66%, 33% respectively) and tumor GPx1 (60%); high levels of tumor Mn-SOD protein expression; tumor MDA (193%) and urinary 8-epi-PGF2 (179%) were observed in serous EOC tumors (M1, n=18) compared with controls (P<0.05). However, higher levels of tumor MDA, Mn-SOD protein expression and urinary 8-epi-PGF2 were observed along with lower tumor CAT activity in poorly differentiated or undifferentiated (grade 3, G 3) versus well or moderately well differentiated (grade 1-2, G 1-2) serous EOC tumors. Obtained data indicate the presence of a severe redox imbalance in EOC and draw attention to the criticial role of AOEs in the pathogenesis of the disease. (c) 2017 IUBMB Life, 69(10):802-813, 2017