The mortality rate of intra-abdominal infections is high, and new prognostic factors are being investigated. Tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-1 beta, and IL-6 are among these factors. Intraoperative peritoneal lavage is part of surgical treatment for intra-abdominal infection. The aim of this study is to investigate the effect of peritoneal lavage on TNF-alpha, IL-1 beta, and IL-6 levels and mortality when the source control has or has not been established. Eighty-five rats were divided into control, cecal ligation and puncture, and cecal ligation and puncture with excision groups. The effect of peritoneal lavage on TNF-alpha, IL-1 beta d, and IL-6 levels and mortality could not be shown in this study.