Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes

Percin I. , ARACAGÖK Y. D. , İDİL N. , DENİZLİ A. , Mattiasson B.

TURKISH JOURNAL OF CHEMISTRY, vol.45, no.5, pp.1353-1365, 2021 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 45 Issue: 5
  • Publication Date: 2021
  • Doi Number: 10.3906/kim-2106-16
  • Page Numbers: pp.1353-1365
  • Keywords: Laccase, N-methacryloyl-(L)-phenylalanine methyl ester, cryogel, textile dye decolorization, reactive blue, IMMOBILIZED LACCASE, WASTE-WATER, POLYMERIC CRYOGELS, SELECTIVE REMOVAL, KINETIC-ANALYSIS, REACTIVE DYES, DETOXIFICATION, DEGRADATION, ADSORPTION, PARTICLES


In this study, amino acid functionalized poly(2-hydroxyethyl methacrylate-N-methacrylolyl-1-phenylalanine) [PHEMAPA] cryogel discs were prepared. In this respect, phenylalanine containing N-methacryloyl-(L)-phenylalanine methyl ester (MAPA) was polymerized with 2-hydroxyethyl methacryl ate (HEMA) without requirement of any activation step. Laccase bound poly(2-hydroxyethyl methacrylate-N-methacryloyl-1-phenylalanine) [Lac-PHEMAPA] cryogel discs were applied for decolorization of Reactive Blue-247 (RB-247). The ability of Lac- PHEMAPA cryogel discs on dye decolorization was found to be as 90% in 2 h and even more within 4h. The decolorization activities of 86% and 73% were observed at relatively low (4 degrees C) and high (60 degrees C) temperatures, respectively. , Ihe effect of dye concentration on dye decolorization and 100% decolorization activity was achieved in dye concentration between 50-300 ppm. Lac-PHEMAPA cryogel discs maintained 80% of its decolorization activity after six cycles. Consequently, the PHEMAPA cryogel discs are promising materials for immobilizing lactase. The Lac-PHEMAPA has a rapid dye decolorization in a broad range of temperature. The preparation is furthermore very stable and activity is preserved during storage.