Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes

Percin I., ARACAGÖK Y. D., İDİL N., DENİZLİ A., Mattiasson B.

TURKISH JOURNAL OF CHEMISTRY, vol.45, no.5, pp.1353-1365, 2021 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 45 Issue: 5
  • Publication Date: 2021
  • Doi Number: 10.3906/kim-2106-16
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.1353-1365
  • Keywords: Laccase, N-methacryloyl-(L)-phenylalanine methyl ester, cryogel, textile dye decolorization, reactive blue, IMMOBILIZED LACCASE, WASTE-WATER, POLYMERIC CRYOGELS, SELECTIVE REMOVAL, KINETIC-ANALYSIS, REACTIVE DYES, DETOXIFICATION, DEGRADATION, ADSORPTION, PARTICLES
  • Hacettepe University Affiliated: Yes


In this study, amino acid functionalized poly(2-hydroxyethyl methacrylate-N-methacrylolyl-1-phenylalanine) [PHEMAPA] cryogel discs were prepared. In this respect, phenylalanine containing N-methacryloyl-(L)-phenylalanine methyl ester (MAPA) was polymerized with 2-hydroxyethyl methacryl ate (HEMA) without requirement of any activation step. Laccase bound poly(2-hydroxyethyl methacrylate-N-methacryloyl-1-phenylalanine) [Lac-PHEMAPA] cryogel discs were applied for decolorization of Reactive Blue-247 (RB-247). The ability of Lac- PHEMAPA cryogel discs on dye decolorization was found to be as 90% in 2 h and even more within 4h. The decolorization activities of 86% and 73% were observed at relatively low (4 degrees C) and high (60 degrees C) temperatures, respectively. , Ihe effect of dye concentration on dye decolorization and 100% decolorization activity was achieved in dye concentration between 50-300 ppm. Lac-PHEMAPA cryogel discs maintained 80% of its decolorization activity after six cycles. Consequently, the PHEMAPA cryogel discs are promising materials for immobilizing lactase. The Lac-PHEMAPA has a rapid dye decolorization in a broad range of temperature. The preparation is furthermore very stable and activity is preserved during storage.