Optimization of monosize/cationic nanoparticle synthesis and their interaction with genomic DNA


Guven G., Piskin E.

POLYMERS FOR ADVANCED TECHNOLOGIES, cilt.17, ss.850-854, 2006 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 17
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1002/pat.840
  • Dergi Adı: POLYMERS FOR ADVANCED TECHNOLOGIES
  • Sayfa Sayıları: ss.850-854

Özet

Monosized poly(styrene/poly(ethylene glycol) ethyl ether methacrylate/N-[3-(dimethyl-amino)propyl]methacrylamide) [poly(St/PEG-EEM/DMAPM)] monosize cationic nanoparticles were produced by emulsifier-free microemulsion polymerization conducted in the presence of a cationic initiator, 2,2'-azobis(2-methylpropionamidine)dihydrochloride (V-50), in which polymerization temperature, time and pH were changed. Particle sizes and surface charge densities were measured with a Zeta Sizer. Atomic force microscopy (AFM) was also used for measurement of particle size. The nanoparticles with the minimum size of 77.6 nm and with quite narrow size distributions (PI = 0.125), and with a zeta potential of about 54.4 mV (cationic) were produced by this method. The optimal temperature, time and pH were 60 degrees C, 3 hr and 2.5-3.0, respectively. It was possible to bind about 200 mg DNA per gram of nanoparticles at room temperature, in 2 hr in the pH range 3-8. Copyright (c) 2006 John Wiley & Sons, Ltd.