Electrochromatographic Enantioseparation of Amino Acids Using Polybutylmethacrylate-based Chiral Monolithic Column by Capillary Electrochromatography


Aydogan C. , DENİZLİ A.

CHIRALITY, cilt.24, sa.8, ss.606-609, 2012 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 24 Konu: 8
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1002/chir.22065
  • Dergi Adı: CHIRALITY
  • Sayfa Sayıları: ss.606-609

Özet

This article describes the development of a polybutylmethacrylate-based monolithic capillary column as a chiral stationary phase. The chiral monolithic column was prepared by polymerization of butyl methacrylate (BMA), ethylene dimethacrylate (EDMA), and N-methacryloyl-l-glutamic acid (MAGA) in the presence of porogens. The porogen mixture included N,N-dimethyl formamide and phosphate buffer. MAGA was used as a chiral selector. The effect of MAGA content was investigated on electrochromatographic enantioseparation of d,l-histidine, d,l-tyrosine, d,l-phenyl alanine, and d,l-glutamic acid. The effect of acetonitrile (ACN) content in mobile phase on electro-osmotic flow was also investigated. It was demonstrated that the poly(BMA-EDMA-MAGA) monolithic chiral column can be used for the electrochromatographic enantioseparation of amino acids by capillary electrochromatography (CEC). The mobile phase was ACN/10?mM phosphate buffer (45:55%) adjusted to pH 2.7. It was observed that l-enantiomers of the amino acids migrated before d-enantiomers. The separation mechanism of electrochromatographic enantioseparation of amino acids in CEC is discussed. Chirality 24:606609, 2012. (c) 2012 Wiley Periodicals, Inc.