Determination of the healthiness of aquaculture fish by enzymes and histopathological methods

Telli-Karakoc F., BARLAS N.

MARINE POLLUTION BULLETIN, vol.149, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 149
  • Publication Date: 2019
  • Doi Number: 10.1016/j.marpolbul.2019.110535
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Endocrine disrupters, Enzymes (EROD, CAT, SOD), Fishes, Metals (i.e. Ni, Cu, Zn, As, Cd, Pb and Hg), Aquaculture
  • Hacettepe University Affiliated: Yes


The health of fishes from select aquacultures was investigated by conducting histopathologic and enzymatic analyses, as well as by examining pollutant accumulation rates in fish tissues ranging in age from juvenile to two years old. Histopathologic examinations demonstrated that the fishes had some abnormalities in their livers, spleens, intestines and reproduction systems, such as lipidation, ovotestis formation, lysis and enlargements of the tissues. The occurrence rate of these abnormalities was not very frequent but also not negligible. Statistical analysis demonstrated that enzyme activity (i.e. CAT, EROD, SOD) and protein concentration fluctuated predominantly by age and season. These parameters were not found to be related to the fish farm or other spatial changes, when their existing environmental conditions were not extreme (i.e. polluted or otherwise unsuitable). Metal concentrations (i.e. Ni, Cu, Zn, As, Cd, Pb and Hg) were never found to be higher than national or international regulatory limits. The quality of the fishes caught from optimal farm conditions may be evaluated as good quality for human consumption.