Voltammetric Determination of Cefuroxime Axetil in Pharmaceuticals Using Graphene Oxide Modified Glassy Carbon Electrode and Hanging Mercury Drop Electrode

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Özaltın N., Erdoğan Kablan S.

Other, pp.463-472, 2018

  • Publication Type: Other Publication / Other
  • Publication Date: 2018
  • Page Numbers: pp.463-472
  • Hacettepe University Affiliated: Yes


Two novel, economic, rapid, simple, sensitive and selective square wave voltammetric (SWV)

methods have been developed for the determination of cefuroxime axetil (CEFA) in pharmaceutical preparations.

Electrochemical reduction and oxidation of the substance on hanging mercury drop electrode and

graphene oxide modified glassy carbon electrode were investigated by square wave voltammetric methods

which employ scan rates up to 1000 mV/s or faster, allowing much faster determinations. For hanging mercury

drop electrode, well-defined peak was obtained at –1.06 V vs. Ag/AgCl/4.6 M KCl in 0.1 M phosphateborate

buffer pH 7.0, limit of detection (LOD), limit of quantification (LOQ) and linearity range were found

0.09, 0.26, and 0.26–15 μg mL–1, respectively. For modified glassy carbon electrode, well-defined peak was

obtained at 1.30 V vs. Ag/AgCl/4.6 M KCl in Britton–Robinson buffer pH 2.0, LOD, LOQ and linearity

range were found 2.70, 8.20, and 8.20–45 μg mL–1, respectively. According to validation studies, the developed

SWV methods were found as accurate, precise, specific, sensitive, repeatable, rugged and robust. The

developed and validated SWV methods were applied to the determination of CEFA in pharmaceutical formulations.

The results were compared with those obtained by a published ultraviolet spectroscopic method and

no difference was found statistically.