Evaluation of the cytotoxicity of different root canal sealers on L929 cell line by MIT assay


YILMAZ Z. , DOĞAN A., OZDEMIR H. O. , SERPER A.

DENTAL MATERIALS JOURNAL, vol.31, no.6, pp.1028-1032, 2012 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 6
  • Publication Date: 2012
  • Doi Number: 10.4012/dmj.2012-172
  • Title of Journal : DENTAL MATERIALS JOURNAL
  • Page Numbers: pp.1028-1032

Abstract

The purpose of this study was to evaluate the cytotoxic effects of commercially available root canal sealers [Sealite Ultra (SU), Tubli-Seal (TS), Tubli-Seal EWT (TS-EWT), Pulp Canal Sealer (PCS), Pulp Canal Sealer EWT (PCS-EWT), Endomethasone N (En N), and Apexit Plus (AP)] on L929 cells by using MTT assay. After incubation with each sealer's extract at 37 degrees C in a humidified air atmosphere containing 5% CO2 for 24 h, MTT (5 mg/mL) in saline was added into each well and further incubated at 37 degrees C for 4 h. Formazan precipitate was dissolved in a buffer containing 23% sodium dodecyl sulfate and 50% N,N-dimethylformamide (pH 4.7). Optical densities of dissolved formazan were read using a microplate spectrophotometer. AP, TS, and TS-EWT showed no cytotoxicity at any dilution tested. Other sealers exhibited some degree of cytotoxicity at the 1/4 and 1/2 dilutions. PCS-EWT and SD exerted more potent cytotoxicity at 1/2 dilution than the other sealers.